FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11296202
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG965310.8545438546513243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC8750.013.58482664
TATACAG31000.013.2552645
TAGGTAG10500.012.710385
GTATATA52900.012.6996361
GTATTAG19050.012.5553121
CCGCACC9500.012.51367865
CTAATAC25800.012.3972133
TACACAG14850.012.2981865
GTATAGA20350.012.2717781
ACTATGC19250.012.2027338
TCTATAC22000.012.1420863
TATACTG28200.012.0805545
GTCCTAA10000.011.9589351
TAGACAG15400.011.630915
CGCACCT9650.011.2334366
ATACAGT27900.011.1915396
TAATACT42950.010.7985014
TATATAC58050.010.5353613
GTGTATA18000.010.3565941
TATACGG16300.010.3423185
Produced by FastQC (version 0.11.5)