FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_bc_anc5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_bc_anc5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647043
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA750.001832649223.33490210
TAGGTAG4300.019.5361965
TCCGCAC2701.2369128E-1018.14606364
TCGGCCG1452.4422686E-416.8946150
CCGCACC2753.085006E-916.54355265
AGCGGGG1704.3920532E-516.46869550
GTATATA25250.016.2287671
GTATTAG7200.015.0796141
CACAGTG4201.8189894E-1215.0010087
TATACAG13900.014.8571145
GCCTAAG1901.1278872E-414.746791
TGTTAGG3655.7298166E-1014.3849662
GCGCGTC1500.00593044914.00221742
CTCCGCA3504.8967195E-914.0009419
TATACTG11000.013.6827385
CGCACCT3354.1698513E-813.58052966
CCCCGCA2901.6639206E-613.27796544
GGCCCCG1850.0015500613.24172211
TAGGACA4558.367351E-1113.0778024
CTAATAC8300.013.0711833
Produced by FastQC (version 0.11.5)