FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_bc_anc4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_bc_anc4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032006
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1656.8046575E-821.2126068
ACGCGGG1351.4131377E-418.14811570
GTGCGTG6300.016.66663732
ATGCGTC6350.016.5345962
GCGCAGC5050.015.93938956
TGCGTAC6850.015.83821951
GTATAGA10950.015.6701721
GTGCGAC5650.015.48936729
CGACGTG5450.015.41281732
GACGTGA5450.015.41281733
GCGTACT7150.015.17367752
AGCTCGC5100.015.09652860
CCGCACC3957.2759576E-1215.06252365
CAGCTCG5350.015.04522259
CGGTCGG1400.003757746214.99849813
GCTCGCG5200.014.8062161
GTGCGCA5450.014.769526554
TGTGCGC5750.014.60759253
GACCGCG1450.00474084614.48308857
CACGCAG6300.014.44299816
Produced by FastQC (version 0.11.5)