FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_bc_anc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_bc_anc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2885921
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC6200.016.371053
CTACCCC4500.015.5575214
TCCTTGC17050.015.1914828
CCCTTGC9100.015.0008548
CCTTGCG9250.014.7573419
CCGCACC5750.014.60699765
GTCCTAG4151.8189894E-1114.344381
CTACACT8800.014.319994
TCCTAGG3454.0017767E-914.2044452
AGGGTGC3252.7981514E-814.0007976
CCTAGGT3001.6008744E-714.000073
TCCGCAC5850.013.75908364
TACGTCC4607.2759576E-1213.6973814
CGCACCT5900.013.64248166
CTAGACT6050.013.3074664
CGGCGGG2659.631984E-613.20601163
CTCCTAG4002.246452E-913.1314371
TATACTG17600.013.1257485
CCCTGCC6400.013.125529
CTAATAC13400.013.0597673
Produced by FastQC (version 0.11.5)