FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_bc_anc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_bc_anc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1656542
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG3351.5643309E-1015.6806691
GTACTAG4301.8189894E-1214.6596021
TACACCG3901.0004442E-1014.3603055
TCCTATA6650.012.6331322
CCGTGTA2254.7482896E-412.4452249
GTAGGGG2254.7519145E-412.4440973
GTATAGA7850.012.0451511
TAGGTAG3501.0387339E-612.0011135
GTGTAGG3003.4152275E-511.6733881
TATACCG4206.258233E-811.6677485
TACACGG2100.00400558411.6677485
TATACAG9050.011.6032865
GTATAGC5156.566552E-1011.5600541
GTATAAG7600.011.5197911
TATAAGG7600.011.5145732
TAAGCCT4903.7362042E-911.429634
CCGTCCC2150.004775762611.3960619
GTCCTAT4351.01110345E-711.2708571
TTAGCCT4401.1922384E-711.1373964
ACTATGC6006.366463E-1111.0840278
Produced by FastQC (version 0.11.5)