FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_bc_anc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_bc_anc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450948
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT4100.017.07164866
TCCGCAC3900.016.15240564
CCGCACC4700.014.14767565
TAGACAG6900.013.6963865
TACACCG4355.7298166E-1012.8742545
GCTTAGG2456.725376E-512.8633451
GTATAGA11150.012.5621091
ACAGCCC4306.6356733E-912.2099588
GTATAAG10950.012.1519771
GTATATA20550.012.09828951
GCGCTCG2356.848168E-411.9155339
GTATTAG9900.011.6722941
TAGGGGT2701.6726599E-411.6672934
GGACCGT3307.074792E-611.6672936
ACCGACC3003.435078E-511.6672928
CCTGGAC3601.4618417E-611.6665783
CTAATAC10850.011.6128153
TACACAG5904.5474735E-1111.27179055
GACCCGT2500.001150235411.2006016
ACTATGC11500.010.95710858
Produced by FastQC (version 0.11.5)