FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741187
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTGC4500.019.4466238
TCCTTGC8950.018.7730528
CTAGGAC2306.0881575E-918.2602923
GCGCACC1953.9515362E-717.9491835
GTCTCCT4350.017.7066941
CTAGACT3002.910383E-1117.5019594
CCTTGCG5250.017.3352769
TCCTATG5450.016.6995982
CTCCTAT5950.016.4757461
CGCGCGG1503.1631452E-416.3314154
CCTTGCC5600.016.251829
GGACCGC1300.002289721316.1556556
CGCACCT3351.5825208E-1015.66979766
CGCGGGA1605.1686383E-415.31069856
CCTCCTA3101.4963007E-814.6820151
CTACCCC3101.5001206E-814.6790644
GCTCGCG2656.0421735E-714.52659112
ACCCCTT4850.014.43460756
CGGGCCG2202.4582823E-514.31649722
GTATTAG7100.014.3002511
Produced by FastQC (version 0.11.5)