FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229168
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGGG200.006950967552.48854419
GGCTAAC350.001248291540.034951
GTACTCC459.167048E-538.897384
TCCGGCT501.704031E-435.0076434
GCCGAGC400.002412820235.007649
CCGCCCG400.002412820235.0076427
CGGCAGC450.0042967431.11790540
CGCCTGA450.0042967431.11790525
CCCGTCG450.0042967431.11790530
GCCCGTC450.0042967431.11790529
CGCCCGT450.0042967431.11790528
ACGTAAC450.004305931331.1043215
TACGTCC604.9656397E-429.1730374
GCCAACC604.9783505E-429.16030364
CGTAAGG500.007183180728.0122262
ACCGCCC500.00719084328.00611326
GTACCTC500.00719851228.0000023
CCTAAAC808.640971E-526.253
ATGGGCA750.001826253623.3384274
CAAGTAG750.00183089923.32824366
Produced by FastQC (version 0.11.5)