FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2889341
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCACT4000.015.74837611
CGCGGGA2505.2746163E-613.99879856
ACCGCGC2456.752212E-512.8580438
TCCTTGC14050.012.45638758
CGGGTCG2000.002784959712.24894918
GACCGCG2601.1767899E-412.1162327
ACTATGC11300.012.0804918
GTATAGA12100.011.8652631
CGAATAG20500.011.78008870
TATACAG15500.011.7431645
GTATAAA19200.011.6723321
CTAGACA8700.011.6678874
CTATACT10500.011.6678874
GTATTAG10550.011.6170121
GTACTAC7550.011.5950311
GCTATAC6950.011.5829433
TATACGG9400.011.543765
ATTATAC19000.011.2370453
TAATACT18400.011.2240014
ATCCTTG19650.011.0440297
Produced by FastQC (version 0.11.5)