FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253408
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2840.1120722313423412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA450.004291688631.1266441
GTGATCG450.00429999131.11435527
ACACGAT500.007203201827.99739615
TTAGAAC1056.747159E-726.6641863
GTCTAAT750.001823802623.3449821
ACTAGTG1253.0734936E-622.4023385
TGTACGG800.002659856321.8772812
ATCAGGC800.002662906421.87296516
AGGATCA800.002662906421.87296513
GTTCTAA1003.9087678E-421.0104831
CTTAGAA1003.919206E-421.0021922
ACGCTTG850.003780448620.5903846
GAGAGGT3450.020.28796870
GTTCCGG1055.432112E-420.0099831
GTATTAA900.005250565719.4541511
ACTTAGA900.005250565719.4541511
GATACTA900.00526256819.4464722
ATTATAG1107.459818E-419.0891323
TGTGCGC950.007199349318.4193383
ACGGGAG950.007199349318.41933817
Produced by FastQC (version 0.11.5)