FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74568
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG980.13142366698852054No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGCG200.006926691652.55
ATCCCAT200.006926691652.543
AGTTCCT200.006926691652.55
GCCGTTT200.006926691652.56
ATACTTT503.437137E-642.06
TATACAA350.001248231340.0000045
AATACTA552.9668715E-431.8181845
GTGTTAA450.004282913631.1111131
TAATACT703.4477038E-530.0000024
AAGCGCA500.00716787527.9999986
AAACAAC750.001817075523.3333346
ATTTCTG950.007149100318.42105165
TAGAGAG1159.960327E-418.26086867
CTTTTAT1000.00960813917.51
GAGAGGT1000.00960813917.569
ATAGAGA1605.082136E-415.312570
GAATAGA1800.001240054613.61111268
AATAGAG1900.001863726812.89473669
GACGAAT3003.333838E-511.66666770
CGAATAG2150.004705524511.39534967
Produced by FastQC (version 0.11.5)