FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393985
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCT459.1883354E-538.890724
ACCCTTG501.7092489E-434.9972087
GCTGATC901.754961E-731.108638
CCGCGCG901.754961E-731.108639
ATCCGGG604.9807434E-429.16434112
TCCTTGC853.9527695E-628.8212288
GTACCTA500.0071852128.0155451
ACCCTAA500.0071852128.0155451
CCTTGGG500.007198586628.0048732
TACATCC500.007203049528.001324
GGGCTGA755.581919E-527.9977666
GAGAGTC500.00720751527.9977657
CGCGGGA657.9537084E-426.92092956
GCGCGGG657.9537084E-426.92092955
ATGCGCA657.9537084E-426.92092953
GCGCGTT1101.0147724E-625.45251511
CGCGCGG700.001225693824.99800554
CCTGGGC851.3075955E-424.703918
GTCGCCT1001.3965933E-524.49804512
GTGTTAG1052.0274932E-523.3462871
Produced by FastQC (version 0.11.5)