FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448466
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1450.00474209714.4826829
GTATTAG10850.013.8776981
TAGGTAG5650.013.6282475
TCCTACG3607.2832336E-913.6118742
GTGCGCG1900.001893519412.894679
TATACTG13450.012.7508635
CCTATAC7050.012.4112833
CCTACAC4357.8835E-912.0689023
CCCTACA4155.3067197E-811.8078912
CTGTGTG4501.3027602E-811.6666069
CTAGACA7250.011.5861454
TACGGTG7100.011.3379697
TAGACCG5259.131327E-1011.3332745
GTATATA20850.011.2524591
CTATACA11200.011.2499414
ACCGTGT3752.3887678E-611.1999428
TATACGG8150.011.16558555
CGCACCT3155.6582787E-511.11014566
CGCGCAT2852.7615475E-411.05392840
ATAGGGG3802.8004379E-611.0525743
Produced by FastQC (version 0.11.5)