FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013525
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2251.7672191E-615.55383454
GTATTAG13000.014.8163791
TAGGTAG6800.013.3833145
GTCCTAC4952.5465852E-1112.7347371
TACACCG5501.8189894E-1212.7281885
TATACTG17750.012.4234285
CCTACAC6200.012.4196293
AACGCGC3407.341241E-712.35157452
CTAATAC13050.012.3374373
TATACAG15500.012.1944255
TCCTTGC15250.012.1646068
TCCTAAG7300.011.9889532
CCTAGGG2356.850786E-411.9151573
CGCGTGC2356.850786E-411.91515732
CTAGGAC5351.0004442E-1011.775963
TAGGACA7750.011.742784
CTATACT12650.011.6213884
TAGGACT5451.382432E-1011.5604634
GTGCTAG7350.011.4352751
ATCCTTG20000.011.3756287
Produced by FastQC (version 0.11.5)