FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66468
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGC150.002214429770.009796
TCTATCG150.002214429770.0097955
ATACTAG200.00689893652.546911
ACACGAC200.00691954152.5073436
CACGACC200.00691954152.5073437
CAGCTCT200.00691954152.50734360
TCACCAG200.006940191652.46783417
GTATCAA552.9478854E-431.8466131
TAAACTA450.004276640231.1154615
TTGTACT450.004276640231.1154614
GATAATA500.007131110428.0250192
GTATAAA500.007131110428.0250191
ATAATAT1000.00958965817.5024473
CCGACGA2004.9653136E-717.50244770
TCCTGTC1503.1179618E-416.32332661
GCCGACG2151.0582316E-616.28134769
TGCCGAC2251.7003113E-615.55773168
CTGCCGA2352.6732087E-614.895699567
GCTGCCG2454.1198437E-614.28771266
ACGCTGC3601.00662874E-712.64065670
Produced by FastQC (version 0.11.5)