FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603255
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG4400.022.2743479
TCCTTGC10200.020.5897318
TCCTAGG2051.4551915E-920.4892922
TAGGTAG4200.020.0014515
GTATTAG7450.019.7391341
CGCGCAT2002.4394467E-819.25319940
ACGCGCA2053.243622E-818.7806839
CGTCCGG1903.008554E-718.42124232
GACGCGC1953.9517E-717.94890238
TCCGCAC3600.017.49799564
CCGAGGG1803.6642523E-617.49799565
CCCTGCC4750.016.9485999
TAGACTG3950.016.8366665
CCCTTGC4600.016.7403478
CAGCGCA3850.016.3648249
CGCGGGA1300.0022930816.15199756
CGCACCT3701.8189894E-1216.07923966
ATCCTTG13550.016.0159237
TCCTAAG4450.015.731482
GTCCGGC2452.47941E-715.71444733
Produced by FastQC (version 0.11.5)