FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_Gbc_c67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_Gbc_c67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783796
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA1801.0913936E-1125.27499456
CCTTGCG6100.022.9521529
CGCGCGG2056.002665E-1122.19267854
TCCTTGC11200.022.1887878
CCTCCTA2451.8189894E-1221.4364261
GTATTAG7550.019.4773371
AACGCGC2405.0386006E-1018.95624552
TAGGTAG4000.018.3760665
CCCTTGC5550.018.289358
CCCGGGG1351.4123668E-418.1492027
CCGGAGC2551.1259544E-917.84667640
CGCACCG2551.1295924E-917.84317236
GGACCCT5750.017.65325
GTATCCT8200.017.5064161
ACCCGGG1200.001340939717.5010176
CGGGGCA1401.865577E-417.5010159
CGGGCCG2702.419256E-916.84999722
TCCGCAC3551.8189894E-1216.75871864
TCCTAAG4200.016.6666982
TCACGGG3156.184564E-1116.66483119