FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046850
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4650.021.0767565
GCGACTC2555.4569682E-1119.21563134
GACGCGC2056.6751454E-717.07354238
CTAGGAC3501.8189894E-1216.9995383
CCTACAC3207.8216544E-1116.4058043
GTATTAG9250.015.9003941
TAGACTG3803.6379788E-1215.6589995
CCTAGCG3201.382432E-915.31545130
CACCGCG2806.8639565E-815.00289128
ACGCGCA2352.7729584E-614.8939439
CTAGACT4051.0913936E-1114.6923944
ACCTAGC3604.656613E-1014.586143529
CTAGACA6350.014.3317194
GTCCTAG3252.779052E-814.007491
CGGTCGG2505.2576343E-614.00269831
CGCGCAT2556.455868E-613.72746440
TAGTCAG4659.094947E-1213.5493429
TCCGCAC3658.85484E-913.42330864
TGGCGCG2102.6519463E-413.3342739
CCGCACC4253.965397E-1013.17514665
Produced by FastQC (version 0.11.5)