FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109464
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1859.968062E-920.812828
GTATTAG8450.016.1626871
TCCGCAC4000.015.74741364
GTATCCT6900.014.7181951
CGCGCAC1708.173124E-414.41315610
CGCACCT4453.6379788E-1214.1549866
CCTATAC5600.013.7500233
GGGAGCG1550.00737239513.5496957
CTGGGGT4404.7293724E-1113.5246734
TAGGTAG4751.2732926E-1113.2650675
CTAGGAC3507.3914634E-813.0000223
CGCGCAT1900.001890908312.89689840
CCGCACC4651.2187229E-1012.79359765
TGACGCG2751.4065916E-512.72880437
GACGCGC1950.002299707212.56531538
ACTATGC9600.012.3970298
CCGCGCA2559.8120065E-512.3541349
GTATTAT18750.012.3267421
GTATATG14700.012.1495021
TATACTG12400.012.1388445
Produced by FastQC (version 0.11.5)