FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533491
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10450.015.750061
GTATATA23700.015.5146371
CCCCGAC1450.004745641314.48108116
TAGGTAG5000.014.0014185
TCCGCAC4303.274181E-1113.83587964
GTATAGA10300.013.2595271
TATACAG13300.012.3696745
CTAATAC12350.012.1877093
ACTGAGC6950.012.0868398
TCTATAC10850.011.9369293
GTATAGG7700.011.8245131
GTATTAT20500.011.7867991
TATAGGG5054.765752E-1011.7847662
TCCTAGA8050.011.741712
CCCAGGC2100.004008103611.6671568
CCTACAC4308.677671E-811.3967283
CTACACT7750.011.2914664
GTACAGG6251.0913936E-1111.2061
CCGCACC5005.14774E-911.19892365
TAGTCAG5351.2478267E-911.12196359
Produced by FastQC (version 0.11.5)