FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1520746
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3150.022.220364
CCGCACC3550.019.71660465
CGCACCT4200.015.83196666
TACACCG3108.8220986E-1015.8066455
GTAGGGC1859.0339214E-515.1358183
CTAATAC8500.014.8241993
TATACGG7550.014.37103755
TATACTG11600.014.1812085
GTCTAAG4202.1827873E-1114.1724331
CCTAAGC2001.753645E-414.0006313
CTATACG3252.7963324E-814.0001714
AGGCGTC2759.1743823E-713.99879120
GGCGTCG2556.459839E-613.726109532
GTCTATA5900.013.649621
GCTCGCG1800.001260421113.60993461
GGCTAGC2851.3719091E-613.50760552
TTATACT11250.013.3779424
GTATAAG7950.013.2129231
CCGCCTA1600.00909315313.12602336
GTGTCCG1600.00909710313.125169
Produced by FastQC (version 0.11.5)