FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2135.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2135.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2455034
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT4050.022.46589366
CCGCACC4100.022.1919265
TCCGCAC4600.019.77975564
CTAATAC14150.014.8406623
ACTATGC11500.014.3049068
GTATATA33300.014.0912361
GTATTAG11950.014.0657151
TATACGG9250.014.0011175
TATACCG8300.013.4950525
GACGCGC2354.5683017E-513.40477838
GTATATG17250.013.1951031
GTATAGA10900.013.17182351
CTATACT11700.013.1634424
CTATGCA10000.012.9505059
CATATAC19550.012.889743
CGCGCAT2203.92324E-412.73062140
ACCTCGC2203.930666E-412.7277688
CCCGTCG2203.937428E-412.72517613
ACGCGCA2254.7506794E-412.44492939
CCTAGCG2852.0252133E-512.28518530
Produced by FastQC (version 0.11.5)