FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2547712
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1656.517923E-414.8503068
ACCTAGG2454.2536412E-614.2925151
GCGCTCG2505.2669457E-614.00061633
CTAATAC10950.013.74327853
CCGTGCG1800.001258843313.6127789
CTAGACT5300.013.2094264
ACCGTGC2659.607236E-613.2091668
CGCTCGT2659.615016E-613.20812934
TCTATAC11450.013.1431363
CTAGGAC4558.367351E-1113.0759583
GCGCACT2952.0239913E-613.04911611
TATACAG15350.012.998595
CTTAGAC5950.012.9402233
CCGAGCG1900.001892854312.89530632
GTATTAG11800.012.7603091
TATACGG8300.012.6524025
TGGACCG3356.115606E-712.5390975
CGCACCG2801.695083E-512.50005970
TAGGTAG6200.012.421125
TATTAGG12200.012.3366092
Produced by FastQC (version 0.11.5)