FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1766279
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC959.3960625E-625.7914248
TCCGCAC2950.022.53961264
GTATTAG7500.019.6092551
TAGGTAG3900.018.8481125
CCTAGCG2053.2354365E-818.78509930
CCGCACC3700.017.9707765
CGCACCT3800.017.49785466
CTGTGCG2051.2589033E-515.3670169
TTAGGTA7850.014.7149054
CTAATAC9300.014.67686753
GCGACTC2505.2661107E-614.00026634
ACCCGCG1750.001018179614.00026533
GTATATA24100.013.9484921
GACTAGG3051.95545E-713.7715348
GTATAGA9000.013.6175391
GCGCGTC1800.001257945213.61368242
TATACAG11800.013.34884555
TCCTATA8150.013.313892
CATACAC5001.8189894E-1213.29953
TATTAGG10550.013.2711452
Produced by FastQC (version 0.11.5)