FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974859
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG2451.8189894E-1221.43361930
CGCGCAT2653.6379788E-1219.81598940
CCGCACC3400.018.52720865
GCGACTC2901.8189894E-1118.10266934
GGGGCTC1803.6590882E-617.50101910
GACGCGC2207.2795956E-817.50013438
GTATTAG11500.017.3589461
TAGGTAG4700.017.1290915
CGCACCT4000.016.6230266
CGATCAC3601.8189894E-1216.5262324
TCCGCAC3601.8189894E-1216.52581264
TACACCG3251.0004442E-1016.1551955
GGCGTCG3753.6379788E-1215.86598432
ACGCGCA2452.4803376E-715.71440539
TAGTCAG5350.015.701859
CTAGGCA3151.1077645E-915.55646110
GTATAGA9000.015.1763891
TATACCG5650.014.8684995
ACCGTGC1901.1340051E-414.73778
GTATAGG7150.014.6947281
Produced by FastQC (version 0.11.5)