FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928994
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT2900.021.7217466
CCGCACC3150.018.88680565
TCCGCAC3250.018.30567264
GTATAGA10150.015.5223691
TAGGTAG5300.015.1893645
TATACAG12600.015.0006775
CTTAGAC5850.014.9571653
GTATATA23750.014.59429551
TATACTG11100.014.1898315
CTAATAC9500.013.99990753
CTCCGTG1550.007375863413.5486489
GTGCGAC2607.880315E-613.46493929
CTAGACT3951.87174E-913.2917394
ACTATGC8700.013.2761178
GTCCTAG3203.498044E-713.1293381
GGGTAGG3203.498044E-713.1293381
CGACGGG1900.001894764612.89331349
GGGCGCA1900.001894764612.89331311
ACGGGCG2456.7613655E-512.85572351
CATATAC11100.012.6125293
Produced by FastQC (version 0.11.5)