FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2076.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2076.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139505
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2600.021.53558564
CTAGGAG1154.1181353E-521.3052433
CCGCACC2650.021.12925365
GTATAGA5800.020.525311
CGGCGCC950.007208817618.42182732
CGCGGCC950.007216121518.41859213
CTTACAC2357.928975E-917.873093
CGCACCT3153.6379788E-1217.77540666
TAGGTAG3005.529728E-1016.3354535
GCGGCCG1300.002292824716.1516914
GTATTAG5550.015.7719661
CTAATAC5600.015.6256563
GTCTAGT3452.4374458E-1015.2233761
GGACAGC1400.00375174915.0012896
TAGGACT1901.1326464E-414.7387564
GTATATA19250.014.5511741
TAGGGGC1450.00473578614.4846384
ATCAGCC4151.8189894E-1114.33543654
TCAGCCA4403.6379788E-1214.3162755
CGCTCGT2707.442941E-714.25985734
Produced by FastQC (version 0.11.5)