FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY2071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY2071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654504
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC2900.024.13562865
TCCGCAC3550.019.71642964
CGCACCT3750.019.5981366
GTATTAG7900.019.500651
CGCGCGG950.00721587818.41929659
TAGGTAG4900.016.4294875
GTCCTAA4350.016.0977171
TAGTCAG4200.015.8342159
ACTATGC7800.015.7060048
GTATATA20900.015.579741
CTAGACT3452.4556357E-1015.2182394
CACGCCC1400.003757233714.998569564
GTGAGCG1656.5195194E-414.8493129
TCCTACG1901.13414455E-414.7372192
TATACAG12400.014.3959635
CTATGCA7350.014.2865119
GTATATG11650.014.1252281
CCTATCC2505.26857E-613.999513
GTATACG6850.013.800561
GTATAGA8150.013.317651
Produced by FastQC (version 0.11.5)