FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_DGY1972.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_DGY1972.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412933
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5250.1271392695667336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCCC755.5684795E-528.008148
TCCGCAC808.6688895E-526.24491564
CTGAGCC750.001827955823.3401179
TATACCC1052.0395819E-523.3288143
CCGCACC901.9284924E-423.32881265
ACTCAGC800.002654177621.8893171
CGCACCT1003.9328868E-420.99593266
CTGTGTG1055.4477266E-420.0058169
CATACTC900.005262397719.4500965
CCTAATA2659.458745E-1118.491462
GTGTTAA1902.9749208E-718.4331071
TTTCCGC1903.001569E-718.41748262
AGTTCCC1150.001004565218.26617810
TCACACA1602.5943713E-517.50508930
ATCACAC1602.5943713E-517.50508929
ACTGTGT1200.001336488217.5050898
GGTGTCC1000.00969097517.4966157
CGATACC1000.00969097517.4966115
CTAATAC1854.7436606E-617.0237273
TAGATAG1452.4291647E-416.9014645
Produced by FastQC (version 0.11.5)