FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-H11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-H11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1258454
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC2000.031.4969664
CCGCACC2250.027.99729765
CGCACCT2750.024.17948566
CCCCGAC1003.9364805E-420.99797257
GCGGCCG1257.8329205E-519.59810814
CTAATAC6450.019.5384333
CCTACAC1852.2680615E-718.9223563
CCGTGTC950.00721168218.4207389
CGCGGCC950.00721498918.41927513
GGGCTGC1351.4127612E-418.1478396
CACCGCG1401.8632645E-417.5031826
GAGCGAC1401.8672322E-417.49831211
CTAGGAC2301.2054079E-716.7421723
ACTATGC5900.016.0166768
ACCGTGT1755.622356E-515.9997278
CTAAGGG2854.9312803E-915.96781353
CTATGCA6000.015.1664099
AACCGTG1656.521578E-414.8482327
TAGGACT1901.13421294E-414.736594
GTATATA19250.014.7352191
Produced by FastQC (version 0.11.5)