FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-H09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-H09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495596
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC2550.021.95827765
TCCGCAC2750.021.63389464
CGCACCT3101.8189894E-1218.06245266
GTATAGG5800.016.3005081
CGACGTG3108.8220986E-1015.80781832
GGTGCGC1350.002944805215.5574218
TAAGGGT5050.015.2493524
CTAATAC7150.015.1746163
GTATAAG6800.014.9332541
GGACAGT3051.21017365E-814.9198226
GTGCGAT1901.1357015E-414.73466711
GTGCGAC3352.6357156E-914.63008829
CCCTAGA2656.001592E-714.53490451
TTCCGCA4600.014.4548763
CTGCTCG1708.17385E-414.412539
CCTAATC2454.2724405E-614.2855163
GTATATA18950.014.22811
GTATTAG5950.014.12406351
GGTGCGA3504.8858055E-914.00308328
GCGCTCG2001.7564859E-413.99793361
Produced by FastQC (version 0.11.5)