FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-H07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-H07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009028
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT2950.022.54009666
GCGCACT2357.954441E-917.87053511
CCGCACC3850.017.27098565
TCCGCAC3950.015.94775764
GTATTAG9300.015.8139081
CCGCGCT1554.0547678E-415.8076135
CCGTCGC2906.2264007E-915.6904149
TATACGG6300.015.5563085
CGGGTCC2505.2585983E-614.00241925
TAGACAG6250.014.0006775
GACCGTC5050.013.8620567
ACCGTCG4303.274181E-1113.8378798
CTATACT9400.013.7772624
TCTATAC9500.013.6318993
CCCGAGC1550.00736851113.5507331
ACCCGCG1550.00737336713.5493833
CTAGGAC3102.386132E-713.5487053
GCGGAGC2405.5585064E-513.12726931
GCCCGGA1600.00910523513.12367550
CATATAC14450.013.0798913
Produced by FastQC (version 0.11.5)