FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407497
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC2203.5106495E-919.0880665
TAGGACA3950.018.6094464
CTAGGAC2306.0899765E-918.2587953
CGCACCT2306.0936145E-918.25814466
CAGCGTG2005.147176E-717.5017419
TCCGCAC2451.3315002E-817.140364
ACCGTGC1653.393669E-516.9713848
ACTCGGG1250.001764619716.79749313
CCGAGGC1300.002293424716.15143613
GTATTAG6950.016.1247331
GCCGCGT2401.9614163E-716.04155233
TATACGG6700.015.6732015
ACACCGT4201.8189894E-1215.00149256
TAGGTAG2806.866503E-815.0014925
CCTAGGA2101.581095E-515.0009592
GGGTCGG1400.003758208414.99776266
TAGGGAG2151.9742083E-514.652625
GTATATA19450.014.584541
GTATATG11400.014.4384441
CGATTCG1951.4164994E-414.35683266
Produced by FastQC (version 0.11.5)