FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762769
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTC1250.001763064316.8000479
TCCGCAC2957.8143785E-915.42289764
CGCACCT2957.8143785E-915.42289766
CTTACAC4600.015.2170033
ACTGATC5400.014.907458
TGCGGGG1656.525241E-414.84768412
CCTAGCG2202.4528948E-514.31984730
GTATAGA8850.014.2433881
GTATAGG5200.014.1406731
GTATTAG8500.014.00600051
GTATATA21750.014.0061
GTCTATA6350.013.7854321
CCCTATA4102.237357E-1013.66092
GCGGGGT1550.007379100713.54765713
TAGACAG4152.7102942E-1013.4940155
CTAATAC8300.013.4936313
GTGTAGG2607.865105E-613.4673071
TAGACTG3901.54796E-913.4615765
GACCGTG2354.5684224E-513.4042947
TAGGTAG4203.2923708E-1013.3333715
Produced by FastQC (version 0.11.5)