FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-H02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-H02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396075
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC2053.2387106E-818.7827173
GTATTAG6350.018.7504431
CTGTGCG1501.49670195E-518.6668769
CCGCACC1953.9552106E-717.94699365
TCCGCAC2108.59427E-716.66506464
TAGACAG4500.016.3341035
TATACCG4300.016.2798375
CAGCCTA1605.162667E-415.3126729
GTCCTAG1858.994363E-515.1434431
CTTAGAC3503.0377123E-1015.00178053
CTGGGGT2101.5813295E-515.0007064
CTTACAC3051.20999175E-814.9198053
TAGGGGT1656.519042E-414.8491844
TAGGTAG3352.6411726E-914.6275555
TATACGG4900.014.2863875
CGCACCT2707.45802E-714.25788866
CTAATAC7400.014.1908743
ACTGTGC3707.021299E-1014.1893498
GTGTAGG2556.4253927E-613.7330241
GTATATA19550.013.6136061
Produced by FastQC (version 0.11.5)