FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084376
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG9500.017.6888871
CGCACCT4250.017.2920566
TAGGACT3601.8189894E-1216.528184
CCGCACC4100.016.21757365
TACACCG3803.6379788E-1215.6582765
GCCGTGC1807.1538256E-515.5559338
CTACACT5200.015.4811454
GACCCGC2101.5845693E-514.99820511
ACCCGCC2101.5845693E-514.99820512
GTATATA23950.014.7638181
GACTCCG2151.976513E-514.651527
TCCGCAC4550.014.61363664
TATACTG12500.014.2803475
CCCGCCT2253.0388386E-513.99832413
CTAGGAC2801.1241245E-613.7493453
CTAATAC10300.013.2517963
ACTATGC9000.012.8336458
GTATATG14000.012.753371
CCTAATA11000.012.7278872
ACAGTGC5501.8189894E-1212.7275828
Produced by FastQC (version 0.11.5)