FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856305
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4300.018.7217965
GTATAGA9050.015.8643521
CTAATAC7750.015.3551343
TAGGACC3452.4556357E-1015.2180944
CGCACCT3302.1409505E-914.8475766
TAGGGTG2656.0316233E-714.5289735
GTATTAG8700.014.4900641
TCTATAC9550.014.2934693
CCTACAC2505.2664473E-614.000273
CCTATAC5300.013.8681913
GTATAGG6900.013.7025591
TAGGCTG2851.3703302E-613.5093965
ATAGGAC3901.54796E-913.4617983
CCGCACC3658.8475645E-913.42383265
TATTAGG9250.013.2452822
CTGATCG2659.614128E-613.2078019
TAATACT15150.013.1689254
CTATACT8800.013.1256064
CTAGGGT1900.001892488312.8953324
TGTAGAC6600.012.7275183
Produced by FastQC (version 0.11.5)