FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811153
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGG1250.001764630916.79785350
GTATTAG7500.016.3402671
TAGGTAG4200.015.8348065
TAGACTG3602.7284841E-1115.5570045
CTGTGCG1951.4139395E-414.36031159
TATACGG6000.013.4179165
GTATAGA8450.013.2600661
ACCGCGC1900.001891789812.8959378
GGGGGCT1900.001891789812.8959379
TAGGCAG3002.4367146E-612.8345285
TACGGTG5501.8189894E-1212.7284577
GTATAGG5551.8189894E-1212.6179681
GTATATA21800.012.5282531
GTCCAGG3407.288472E-712.3581861
ACATAGG4104.4718035E-811.95365231
GTCCTAG2356.8253535E-411.9199511
GTATTAA10000.011.9050521
CCGGCAG2952.892153E-511.86485639
TAATACT13400.011.7548264
TATACTG11650.011.7178295