FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-F09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-F09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723916
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3400.018.52752366
CCGCACC3750.017.73152565
GCCCTAG1602.5985892E-517.5078641
ACACGCG1000.00968987517.50075336
TCCGCAC3700.017.02529164
CTATGCA6750.016.5928279
TAGGACC3108.8220986E-1015.8071344
TAATACT12850.015.5259614
TATACCG5450.015.4135085
TCGCCCC1605.15547E-415.315824525
ACTATGC7650.014.6411528
CGCCCCT2403.4425775E-614.586526
CTAATAC7850.014.266893
AGCGGGG1800.001260964113.60932850
CTAGGGT2659.610812E-613.2081174
ACAGAGC5051.8189894E-1213.1688838
ATACCGT7450.013.1549296
TATATAC19900.013.01450353
CTAGGAT3801.5546902E-812.8941723
GTATATG10950.012.7911341
Produced by FastQC (version 0.11.5)