FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791427
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2103.6379788E-1223.3313954
CGCGGGA2201.5643309E-1020.68009456
GACGCGC2750.020.36421438
CTTAAGG4000.018.3765473
ACGCGCA3002.910383E-1117.50049639
GTATTAG6650.017.377161
CCCCTAG1250.001757189616.8084531
GTATCCT6050.016.7853011
TGACGCG3601.8189894E-1216.52824637
CGCGCAT3207.8216544E-1116.40854640
GTCCTAG2351.535409E-716.391221
CGTCCGG2351.5442129E-716.38298432
AGGTAGC3451.4551915E-1116.2323447
CGGGAAC3009.760697E-915.16540358
CTTCCTA5600.015.0075461
GTCCTAC2806.836126E-815.0075461
AACGCGC3403.263267E-914.41056352
GGTAGCC3901.0186341E-1014.3593828
ACGCGCG2951.3042336E-714.23610153
GTCCTAA3758.54925E-1014.0070431
Produced by FastQC (version 0.11.5)