FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-F05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-F05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910777
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3400.018.52858266
CCGCACC2851.2732926E-1118.42022565
TCCGCAC3203.6379788E-1217.49921664
TATACGG8150.017.1775935
CTAATAC8100.016.8519783
TACGGTG6800.016.470287
GTATATA23750.015.6281221
ACGGGTC1656.5238547E-414.84820658
CATATAC15000.014.700113
ACGGTGT7200.014.583068
TCCTATA7750.014.4532342
GTATTAG8500.014.4182871
CCTAATA9350.014.2261952
GTCTTAC4700.014.1553391
TGCGCGC1800.001260232413.610520
TATATAC26100.013.5441633
ATACGGT8750.013.1997536
CGACGGG1600.00910164913.12441349
CGGTCCG1600.00910164913.12441356
TAGGTAG3751.2914825E-813.0664225
Produced by FastQC (version 0.11.5)