FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-F03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-F03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767976
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10500.015.336481
TAGGTAG6450.014.6520515
ACTATGC11050.013.9374978
CTATACT10550.013.9344944
TATACTG15400.013.8644775
GTCCGAC1800.001261025113.60972411
CGCACCT5500.013.36227466
TAGACAG8400.012.5007585
CTGATCG2852.0317655E-512.2814479
CGACTCC11200.012.18691870
GGAGCGC2356.857616E-411.9136822
CCTATAC7650.011.8952873
ATTATAC21250.011.85868553
GTATAGG9750.011.8485841
CATATAC16400.011.7376693
CGGTCGG2100.004012575411.66547913
TATACAG15650.011.6300985
ACAGTCC5501.6189006E-1011.455248
TATTAGG12950.011.3516292
CAGGACC4954.3892214E-911.3138174
Produced by FastQC (version 0.11.5)