FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717950
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA2504.1836756E-1119.59797756
CGCGCGG2254.642061E-918.66473854
TCCTTGC11500.015.2183398
CCTCCTA3101.4963007E-814.6829261
CGTCCGG3151.8562787E-814.44454832
CTCCTAA5500.014.0052531
CCCCTAC4555.456968E-1213.8513491
ACTATGC11400.013.8166498
CCTTGCG7900.013.7350349
GTATCCT8450.013.6737681
GGTCGCG1550.00738286813.54698811
GTATTAG9050.013.5409911
GTCTTAC5950.013.5344891
CCCCCTG2102.6522196E-413.3344092
ACGCGCG4002.2664608E-913.12364453
GTCTCCT6150.013.0943421
CCTAGAC5950.012.9407933
CGCGCCG3254.247504E-712.921741515
TACTATG12200.012.9106417
TAATACT17100.012.8957794
Produced by FastQC (version 0.11.5)