FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024720
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG1154.1065945E-521.3143631
CCGCACC4200.017.49871665
CGCACCT4400.015.90792366
CGGGGCA1554.0547398E-415.8076344
TCCGCAC4450.015.72918264
TATACCG6050.015.0420755
CCTATAC5600.015.00001053
CTAATAC9350.014.9732723
GTATAGG5150.014.9584861
ACCGTGC2604.864323E-714.8080688
ACTATGC8500.014.4121318
CTATACT9150.014.1540654
CTTACAC4950.014.1414243
TATACTG12000.013.709025
CCGCTCC1800.001259609813.6114589
TAATACT16900.013.4625454
GTATATA22400.013.4438181
TATACGG6000.013.4173385
CAGCGTG4203.2923708E-1013.3336729
AGCGTGT5001.8189894E-1213.30033810
Produced by FastQC (version 0.11.5)