FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250800
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG4100.019.6396331
CGCCGGA900.005282017419.44132412
GGCTCGC950.007204321718.4239887
TAATACG3401.0913936E-1116.4732134
CCTAGGA1350.002946809615.5555472
GTATATA16450.015.1106021
TATACGG4750.014.7391915
GTATAGA6250.014.5640671
CTACCCC1450.004735214.4850664
GGGAGTG1951.4125893E-414.3612627
GTGTATA6850.014.3105661
TACACCG2454.263431E-614.28799255
GTATAAC5300.013.8717991
TACCCCT2303.7250975E-513.6978365
ACAGCCC2303.7250975E-513.6978368
CTGTGCG1550.007367125213.5505479
TATACAG8900.013.3729185
CAAGGGC3152.8915747E-713.3354584
GTATAGG3951.8626451E-913.2948521
CGGGGTT1850.001550228713.241117516
Produced by FastQC (version 0.11.5)