FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178467
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGC700.001227487924.99680366
TCCGCAC1703.765308E-922.64416164
CCGCACC1901.3569661E-820.26056765
CTGTGCG1055.463567E-420.0016869
TAGACCG2152.6284397E-919.536535
CGCACCT1801.7052298E-719.44195766
GCGGGGA1501.4981746E-518.6642851
CGGGGTC950.007216030318.41869713
CCTACAC1953.9534643E-717.9471843
GTATTAG4950.017.683511
CATATAC10050.017.4114483
TATACGG4650.017.3132865
TACGGTG4650.016.5605357
AGCGGGG1704.3908352E-516.46848150
ATACGGT5000.016.1013576
GTATATA16000.015.9748411
TATACAG8800.015.9104325
GTGTATA6500.015.621341
AGACCGT2755.5031705E-815.2740146
TCGCGGT1400.003749773115.00253945
Produced by FastQC (version 0.11.5)