FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120640
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1003.9291786E-421.0034544
CCTGGAC1551.984008E-518.065073
TATACAG9200.017.1223815
CTTACAC2151.0969707E-616.2795683
TAGGACT1300.002288235116.1565044
CCTATAC3401.9645086E-1015.4416493
CTAGTAC3452.4556357E-1015.2178563
ACGGTGT4009.094947E-1214.8767848
CCTACAC1656.5184344E-414.848943
TACGGTG3604.638423E-1014.5850827
GTATAGG4101.4551915E-1114.5152311
ACCGTGC1450.00473603414.4844948
ATACGGT4351.8189894E-1214.4844946
TACAGGC2901.0543954E-714.4844947
ATCCGAC1450.004744462714.48061760
TCCTATA4850.014.4334312
ACCGTGT1708.1674627E-414.4134928
ATACAGG5350.014.3942486
GTGTATG4151.8189894E-1114.3403471
ACTGATC3707.003109E-1014.190898
Produced by FastQC (version 0.11.5)