FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2344559
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10300.018.0177461
CCGGCCC1755.6300792E-515.99826413
TAGGTAG6450.015.7370775
GTCCTAA4800.015.3193331
TATACAG15050.014.884335
CCTAGGC1750.001018022114.0008713
ACATAGG6050.013.88753131
TCGGGGG2102.656225E-413.33188616
CGACGTG4751.2732926E-1113.26341632
CGCGCGG1850.001550325313.24180754
CTAGGAC3456.119808E-813.1892263
GGTGCGA4801.4551915E-1113.12805728
AGCTCGC4002.2664608E-913.12357760
TATTAGG12600.013.0577592
GTGCGAC4355.711627E-1012.8765629
GCTCGCG4406.89397E-1012.72589361
CCCCTGA3356.092432E-712.5429091
GAGTCCG3103.5221692E-612.4195979
GGACAGC3701.5259138E-712.2975376
GCGCTCG2852.0349818E-512.27936961
Produced by FastQC (version 0.11.5)