FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858010
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAC1205.7154157E-520.4167182
TGAGTCG1257.809249E-519.6046228
GCAGCGC900.00526861219.4490288
CTAGGGT900.00526861219.4490284
TATAGGG1702.1184514E-618.5294592
CGCTCGA950.007199353518.42539610
GCGCGAA1200.001342241917.49698411
ACAGGAC2009.935426E-615.7537138
TCCTAAG2452.4757173E-715.7143252
CCTCCTA1350.002937739715.5619421
CAGCGCA1400.003747008715.0035349
TGCGCGA1400.003747008715.00353410
CGTGAGC1400.00375719414.99741516
GTCCTAA2151.9666417E-514.6571791
ACAGCGT2151.969894E-514.6546158
CCTTGCG2656.0077946E-714.5317269
CTGCACA1450.00473119414.4861724
TAGGGCT2001.7494074E-414.0033014
TAGACAG3252.7825081E-814.00335
AGCGCAC1500.00592435414.00329910
Produced by FastQC (version 0.11.5)