FastQCFastQC Report
Thu 20 Dec 2018
HFWYLAFXY_n02_AP3-C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFWYLAFXY_n02_AP3-C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662410
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3255.456968E-1217.2290664
CCTATAC4450.016.5167053
CCGCACC3401.0913936E-1116.46895465
CAGCGTG3057.003109E-1016.0659129
GGCTATA3602.7284841E-1115.5661841
TAGGTAG3151.1077645E-915.5563525
CTACCCC1807.150792E-515.5563524
CGCACCT3602.7284841E-1115.55401166
TACACTG5250.015.3341175
CTAGGGT2302.2162112E-615.2181714
CTATACT7600.015.1981454
CGCGTGC1400.003754333615.00031610
CATATAC12200.014.9178983
CTAATAC8100.014.6912253
GTATATA16950.014.6707861
TCTATAC8150.014.6010943
CTGCGCA2403.4494115E-614.5836419
TATACTG11150.014.44025
ATACGGT6700.014.1047746
ATTAGAC7050.013.9005833
Produced by FastQC (version 0.11.5)